Now available through Stores: BD Falcon 5mL filter-cap tubes. Stores cat. #15007950. $29.77/bag

The Cytometry Core Facility, operated and administered as a partnership between Arizona Research Laboratories, Division of Biotechnology and the Arizona Cancer Center, offers instrumentation to analyze and quantify the characteristics of cells and particles in suspension as they stream in single file past a laser beam.

Four instruments are available for use:

The FACScan (BD Biosciences, San Jose, CA) is an analyzer capable of acquiring and analyzing data from five parameters that include: Relative size, granularity or cell complexity and three fluroescence channels. The excitation source is a 15mW Argon Laser tuned to 488nm.

The FACSAria (BD Bioscienes, San Jose, CA) is a cell sorter equipped with 3 lasers: 488nm, 407nm and 633nm, and offers 9 fluorescence detectors. The sorting function of this instrument enables the researcher to physically separate the individual cells and particles from a mixed population. Viable cells can be recovered for further study or culture. The addition of a Baker BioProtect III biosafety cabinet, in which the Aria is housed, allows for sorting of certain live human tissue and BSL2 agents.

The LSRII (BD Biosciences, San Jose, CA) is an analyzer capable of acquiring and analyzing data from 14 fluorescence parameters and has four lasers: 405nm, 488nm, 532 nm and 640nm.

The Reflection (iCyt, Champaign, IL) is a Special Project cell sorter with ten fluorescence parameters and four lasers: 355nm, 405nm, 488nm, and 640nm.

We also offer information about new techniques, instrumentation and applications of flow cytometry provided through Biotechnology-sponsored workshops and seminars.

Individual consultation services are available to discuss applications and methodologies specific to your project.